Preheat the air fryer to 350°F.
In a small bowl, whisk sour cream, lime juice, chili powder, garlic powder, salt, and pepper.
Spray the air fryer rack with cooking spray.
Spread the sauce evenly on one tortilla and place it sauce-side up in the air fryer.
Add chicken and cheese evenly on top.
Cover with the second tortilla and lightly spray the top.
Secure with toothpicks to keep it in place.
Cook the Air Fryer Chicken Quesadilla for 8 minutes, flipping halfway through.
Let cool slightly, slice into quarters, and serve.